In the coming decade, NASA's Artemis campaign is set to explore the Moon for scientific discovery and technological advancement and to learn how to live and work on another world as we prepare for human missions to Mars. However, our biology faces numerous risks associated with leaving Earth's atmosphere. Engineering biology offers a unique toolkit for addressing these challenges, ranging from protection against radiation and longevity enhancement to the on-demand manufacturing of molecules essential for sustenance, energy, and shelter during deep space exploration and planetary missions. In this session, NASA Astronaut Kate Rubins, PhD, and Christopher Mason, PhD will discuss the roadmap for engineering biology to enable space settlement