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Research Associate/Scientist-Molecular Biology, Conagen Inc., Bedford, MA

Our company:
Conagen Inc. is a leader in cutting-edge bio-manufacturing. We engineer organisms to produce high-value molecules that are essential to our well-being. Our focus is on food, nutrition, pharmaceuticals, and renewable materials. We employ the latest synthetic biology tools to design metabolic pathways and produce safe and high-quality products. Science and innovation are the driving forces of our mission. We believe in a green future.

Location: Bedford, MA

Job Type: Full-time

Compensation: Commensurate with skills and experience

Job Description Overview:
We are currently seeking a research associate/scientist to support projects related to identification and functional characterization of metabolic enzymes for metabolic pathway engineering projects. The candidate is expected to have a strong background in molecular biology, biochemistry, metabolic engineering and other related disciplines. Strong technical, organizational, and communication skills, as well as the ability to develop creative solutions to challenging problems, are essential.

Responsibility:

Routinely perform molecular biology techniques such as primer design, PCR, DNA and RNA extraction, DNA mutagenesis, DNA cloning, plasmid mini-prep, DNA and protein sequence analysis, cell transformation, cell culture, and other more advanced molecular biology techniques in support of research projects.
Utilize sequence analysis software such as Geneious or Benchling or and some basic bioinformatics tools.
Perform experiments for enzymatic activity testing including express proteins in E. coli and yeast, recombinant proteins purification; analyze expressed proteins by SDS-PAGE and/or Western Blot and in vitro/in vivo enzymatic assays.
Operate laboratory and experimental equipment such as spectrophotometers, centrifuges, HPLC, FPLC, LC/MS and GC/MS.
Strain engineering to optimize production of desired compounds and protein.
Optimize the cultivation condition and assays for strains screening
Work collaboratively with other team members to accomplish project goals and milestones.
Routinely track and document results in laboratory notebooks and databases.
Analyze, summarize, and present experimental results to team leader and other team members.
Qualification:

A Master degree with 2+ years’ experience or a Ph.D. degree in biochemistry, metabolic engineering, molecular biology, microbiology, or other related fields.
Strong background in protein engineering, metabolic engineering, molecular biology, genetics, and synthetic biology with experience in recombinant DNA technologies, protein analysis, enzymatic assays, and cell transformation.
Experience in engineering of metabolic pathways in microbial hosts is highly preferred.
Familiarity with analytical techniques for metabolite detection such as spectrophotometers, HPLC, LC/MS and GC/MS.
Familiarity with diverse software packages for data processing and statistical analysis, primer design and sequence blasting and alignment.
Must be highly motivated and able to work independently while also possessing exceptional communication and teamwork skills.

Equal Employment Opportunity:
We are proud to be an equal opportunity employer – and celebrate our employees’ differences, regardless of race, color, religion, gender, sexual orientation, gender identity, national origin, age, disability, or Veteran status.

 

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Anissa Cooke

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